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Tech Tip Videos to Improve Your Next Experiment

How much antibody to use in ChIP assays?

More antibody isn't always better! See how the amount of antibody used can affect your Chromatin Immunoprecipitation (ChIP) results and signal-to-noise, and how to adjust your IP. How much antibody to use in ChIP assays?

Better IHC Step 1: Antigen Retrieval

We'll show you why antigen retrieval is important for immunohistochemistry and give you tips on how to set up heat induced epitope retrieval in your microwave. Better IHC Step: 1 Antigen Retrieval

Formaldehyde vs Alcohol Fixation for IF

Let's explore the difference between fixatives: crosslinking formaldehyde vs methanol and other alcohols for immunofluorescence (IF).  Formaldehyde vs Alcohol Fixation for IF

Characterize Immune Responses with Flow Cytometry

Combining analysis of surface markers and intracellular signaling readouts through multiplexed flow cytometry adds power to your experiments. This can present challenges when designing antibody panels. This video walks through an example experiment to illustrate how antibodies can be selected for a panel to assess activation of T cell subpopulations: How to Characterize Immune Responses with Flow Cytometry

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